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KMID : 1098420060140010031
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Korean Journal of Medicinal Crop Science
2006 Volume.14 No. 1 p.31 ~ p.35
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Plant Regeneration from Hairy Root of Rehmannia glutinosa Liboschitz Transformed by Agrobacterium rhizogenes
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Hwang Sung-Jin
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Abstract
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A protocol for plant regeneration from hairy root of Rehmannia glutinosa transformed by Agrobacterium rhizogenes ATCC15834 has been developed. Transgenic shoots were regenerated from hairy roots within 6 weeks after culture on the SH medium supplemented with 0.5 mg/l BA. Shoots were rooted on plant growth regulator free SH medium successfully. The transformed plants, which were regenerated from hairy roots, had thiner roots with extensive lateral branches, wrinkled leaves, shorter node, and grew faster compared with non-transformed plants. The biomass of the transformed plant was 1.28 g (F.W) per plant, significantly higher than the non-transformed plant (0.54 g F.W). The catalpol content in the transformed plant (0.56%) was also higher than that of the non-transformed plants (0.43%).
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KEYWORD
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hairy root, plant regeneration, catalpol, Rehmannia glutinosa
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